Expression, purification and crystallization of the SARS-CoV macro domain.
Identifieur interne : 000306 ( France/Analysis ); précédent : 000305; suivant : 000307Expression, purification and crystallization of the SARS-CoV macro domain.
Auteurs : Hélène Malet [France] ; Karen Dalle ; Nicolas Brémond ; Fabienne Tocque ; Stéphanie Blangy ; Valérie Campanacci ; Bruno Coutard ; Sacha Grisel ; Julie Lichière ; Violaine Lantez ; Christian Cambillau ; Bruno Canard ; Marie Pierre EgloffSource :
- Acta crystallographica. Section F, Structural biology and crystallization communications [ 1744-3091 ] ; 2006.
Descripteurs français
- KwdFr :
- Clonage moléculaire, Cristallisation, Diffraction des rayons X, Fragments peptidiques (), Fragments peptidiques (isolement et purification), Protéines recombinantes (), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Protéines virales (), Protéines virales (génétique), Protéines virales (isolement et purification), Protéines virales (métabolisme), Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (isolement et purification), Protéines virales non structurales (métabolisme), RNA replicase (), RNA replicase (génétique), RNA replicase (isolement et purification), RNA replicase (métabolisme), Virus du SRAS (enzymologie).
- MESH :
- enzymologie : Virus du SRAS.
- génétique : Protéines virales, Protéines virales non structurales, RNA replicase.
- isolement et purification : Fragments peptidiques, Protéines recombinantes, Protéines virales, Protéines virales non structurales, RNA replicase.
- métabolisme : Protéines recombinantes, Protéines virales, Protéines virales non structurales, RNA replicase.
- Clonage moléculaire, Cristallisation, Diffraction des rayons X, Fragments peptidiques, Protéines recombinantes, Protéines virales, Protéines virales non structurales, RNA replicase.
English descriptors
- KwdEn :
- Cloning, Molecular, Crystallization, Peptide Fragments (chemistry), Peptide Fragments (isolation & purification), RNA Replicase (chemistry), RNA Replicase (genetics), RNA Replicase (isolation & purification), RNA Replicase (metabolism), Recombinant Proteins (chemistry), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (enzymology), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (isolation & purification), Viral Nonstructural Proteins (metabolism), Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (isolation & purification), Viral Proteins (metabolism), X-Ray Diffraction.
- MESH :
- chemical , chemistry : Peptide Fragments, RNA Replicase, Recombinant Proteins, Viral Nonstructural Proteins, Viral Proteins.
- chemical , genetics : RNA Replicase, Viral Nonstructural Proteins, Viral Proteins.
- chemical , isolation & purification : Peptide Fragments, RNA Replicase, Recombinant Proteins, Viral Nonstructural Proteins, Viral Proteins.
- chemical , metabolism : RNA Replicase, Recombinant Proteins, Viral Nonstructural Proteins, Viral Proteins.
- enzymology : SARS Virus.
- Cloning, Molecular, Crystallization, X-Ray Diffraction.
Abstract
Macro domains or X domains are found as modules of multidomain proteins, but can also constitute a protein on their own. Recently, biochemical and structural studies of cellular macro domains have been performed, showing that they are active as ADP-ribose-1''-phosphatases. Macro domains are also present in a number of positive-stranded RNA viruses, but their precise function in viral replication is still unknown. The major human pathogen severe acute respiratory syndrome coronavirus (SARS-CoV) encodes 16 non-structural proteins (nsps), one of which (nsp3) encompasses a macro domain. The SARS-CoV nsp3 gene region corresponding to amino acids 182-355 has been cloned, expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 37.5, b = 55.6, c = 108.9 angstroms, beta = 91.4 degrees, and the asymmetric unit contains either two or three molecules. Both native and selenomethionine-labelled crystals diffract to 1.8 angstroms.
Url:
DOI: 10.1107/S1744309106009274
PubMed: 16582497
Affiliations:
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Links to Exploration step
pubmed:16582497Le document en format XML
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et Fonction des Macromolécules Biologiques, UMR 6098-Case 932, 163 Avenue de Luminy, 13288 Marseille CEDEX 9</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Dalle, Karen" sort="Dalle, Karen" uniqKey="Dalle K" first="Karen" last="Dalle">Karen Dalle</name></author><author><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name></author><author><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></author><author><name sortKey="Blangy, Stephanie" sort="Blangy, Stephanie" uniqKey="Blangy S" first="Stéphanie" last="Blangy">Stéphanie Blangy</name></author><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name></author><author><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name></author><author><name sortKey="Grisel, Sacha" sort="Grisel, Sacha" uniqKey="Grisel S" first="Sacha" last="Grisel">Sacha Grisel</name></author><author><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name></author><author><name sortKey="Lantez, Violaine" sort="Lantez, Violaine" uniqKey="Lantez V" first="Violaine" last="Lantez">Violaine Lantez</name></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name></author><author><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie Pierre" last="Egloff">Marie Pierre Egloff</name></author></analytic><series><title level="j">Acta crystallographica. Section F, Structural biology and crystallization communications</title><idno type="eISSN">1744-3091</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term><term>Crystallization</term><term>Peptide Fragments (chemistry)</term><term>Peptide Fragments (isolation & purification)</term><term>RNA Replicase (chemistry)</term><term>RNA Replicase (genetics)</term><term>RNA Replicase (isolation & purification)</term><term>RNA Replicase (metabolism)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (enzymology)</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (isolation & purification)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (isolation & purification)</term><term>Viral Proteins (metabolism)</term><term>X-Ray Diffraction</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Clonage moléculaire</term><term>Cristallisation</term><term>Diffraction des rayons X</term><term>Fragments peptidiques ()</term><term>Fragments peptidiques (isolement et purification)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales ()</term><term>Protéines virales (génétique)</term><term>Protéines virales (isolement et purification)</term><term>Protéines virales (métabolisme)</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (isolement et purification)</term><term>Protéines virales non structurales (métabolisme)</term><term>RNA replicase ()</term><term>RNA replicase (génétique)</term><term>RNA replicase (isolement et purification)</term><term>RNA replicase (métabolisme)</term><term>Virus du SRAS (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptide Fragments</term><term>RNA Replicase</term><term>Recombinant Proteins</term><term>Viral Nonstructural Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA Replicase</term><term>Viral Nonstructural Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Peptide Fragments</term><term>RNA Replicase</term><term>Recombinant Proteins</term><term>Viral Nonstructural Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA Replicase</term><term>Recombinant Proteins</term><term>Viral Nonstructural Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Fragments peptidiques</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines recombinantes</term><term>Protéines virales</term><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Crystallization</term><term>X-Ray Diffraction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Cristallisation</term><term>Diffraction des rayons X</term><term>Fragments peptidiques</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Macro domains or X domains are found as modules of multidomain proteins, but can also constitute a protein on their own. Recently, biochemical and structural studies of cellular macro domains have been performed, showing that they are active as ADP-ribose-1''-phosphatases. Macro domains are also present in a number of positive-stranded RNA viruses, but their precise function in viral replication is still unknown. The major human pathogen severe acute respiratory syndrome coronavirus (SARS-CoV) encodes 16 non-structural proteins (nsps), one of which (nsp3) encompasses a macro domain. The SARS-CoV nsp3 gene region corresponding to amino acids 182-355 has been cloned, expressed in Escherichia coli, purified and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 37.5, b = 55.6, c = 108.9 angstroms, beta = 91.4 degrees, and the asymmetric unit contains either two or three molecules. Both native and selenomethionine-labelled crystals diffract to 1.8 angstroms.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><noCountry><name sortKey="Blangy, Stephanie" sort="Blangy, Stephanie" uniqKey="Blangy S" first="Stéphanie" last="Blangy">Stéphanie Blangy</name><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><name sortKey="Dalle, Karen" sort="Dalle, Karen" uniqKey="Dalle K" first="Karen" last="Dalle">Karen Dalle</name><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie Pierre" last="Egloff">Marie Pierre Egloff</name><name sortKey="Grisel, Sacha" sort="Grisel, Sacha" uniqKey="Grisel S" first="Sacha" last="Grisel">Sacha Grisel</name><name sortKey="Lantez, Violaine" sort="Lantez, Violaine" uniqKey="Lantez V" first="Violaine" last="Lantez">Violaine Lantez</name><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Malet, Helene" sort="Malet, Helene" uniqKey="Malet H" first="Hélène" last="Malet">Hélène Malet</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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